膜型セリンプロテアーゼMT-SP1 によるCDCP1 切断を介した癌細胞の足場非依存性能の解析

Abstract

CUB domain-containing protein 1 (CDCP1) is an important molecule for regulating anchorage-independence involved in cancer metastasis. Recently, it was reported that the synthetic substrate of CDCP1 is cleaved by Membrane-type serine protease 1(MT-SP1), however it is not clear that this cleavage is involved in anchorage-independence. In this study, we investigated the effect of CDCP1 cleavage by MT-SP1 on anchorage-independence in cancer cells by soft agar assay using MT-SP1 siRNA and uncleaved CDCP1 mutant (2RKAAres-F). As a result, cleavage of CDCP1 was inhibited in both experiments, and then the anchorage independence was suppressed. Therefore, it is suggested that the cleavage of CDCP1 by MT-SP1 is involved in the regulation of anchorage independence in cancer cells.